荧光淬灭
荧光淬灭(英語:)猝滅是當常溫物質經射線照射(通常為紫外線或X射線)所發出會螢光強度降低的任何過程。
淬滅受壓力和溫度的影響很大,許多過程都可導致淬滅,例如激發態反應,能量轉移,複合物形成和碰撞淬滅。氧分子,碘離子和丙烯酰胺是常見的化學猝滅劑[1] 。眾所周知的奎寧熒光猝滅劑則是氯離子。[2][3][4]
有一系列效应会导致荧光淬灭,例如:
- 复杂结构
- 内部转换
- 能量转移到其他分子,所谓的灭剂
參考文獻
- Acrylamide and iodide fluorescence quenching as a structural probe of tryptophan microenvironment in bovine lens crystallins. Phillips SR, Wilson LJ, Borkman RF. Curr Eye Res. 1986 Aug;5(8):611-9.
- Fluorescence experiments with quinine James E. O'Reilly J. Chem. Educ., 1975, 52 (9), p 610 doi:10.1021/ed052p610
- Photophysics in a disco: Luminescence quenching of quinine LouAnn Sacksteder , R. M. Ballew , Elizabeth A. Brown , J. N. Demas , D. Nesselrodt and B. A. DeGraff J. Chem. Educ., 1990, 67 (12), p 1065 doi:10.1021/ed067p1065
- Halide (Cl-) Quenching of Quinine Sulfate Fluorescence: A Time-Resolved Fluorescence Experiment for Physical Chemistry Jonathan H. Gutow J. Chem. Educ., 2005, 82 (2), p 302 doi:10.1021/ed082p302
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